qPCR ΔΔCt Calculator
Compute relative expression from target and reference Ct values.
Ready when you are.
Enter your values and select Calculate to see your result here.
Compute average Ct, ΔCt, ΔΔCt and the 2^-ΔΔCt relative expression fold change from control and treatment data.
How it works
Method: ΔCt = Ct(target) − Ct(reference). ΔΔCt = ΔCt(treated) − ΔCt(control). Fold change = 2^(−ΔΔCt).
Example: If the control ΔCt is 4 and treated ΔCt is 2, then ΔΔCt = −2 and relative expression is 4-fold.
Cite this toolCopy · BibTeX · RIS
If you use this calculator in research, you may cite the software and report the statistical methods separately. Citation is appreciated, not required. No DOI is registered for this release.
Reproducibility: download the version 1.6 source and tests · SHA-256 checksum. Future updates must retain this archive.
A citation cannot replace a methods description or independent verification. Report version, statistical assumptions and data-processing choices.