qPCR ΔΔCt Calculator
Compute relative expression from target and reference Ct values.
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Compute average Ct, ΔCt, ΔΔCt and the 2^-ΔΔCt relative expression fold change from control and treatment data.
How it works
Method: ΔCt = Ct(target) − Ct(reference). ΔΔCt = ΔCt(treated) − ΔCt(control). Fold change = 2^(−ΔΔCt).
Example: If the control ΔCt is 4 and treated ΔCt is 2, then ΔΔCt = −2 and relative expression is 4-fold.
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